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. 2012 Feb 17;7(2):e30604. doi: 10.1371/journal.pone.0030604

Figure 1. DRE induces apoptosis in CMML cells in a dose dependent manner.

Figure 1

MV-4-11, HL-60 and U-937 cells were treated with increasing doses of DRE and analyzed for the induction of apoptosis. (a) Hoechst and Annexin-V staining of DRE-treated CMML cells 48 hours after treatment. Magnification 400×. (b) Manual quantification of three individual experiments of DRE-treated cells, 48 hours after. 6 pictures were obtained for every sample and live versus dead cells were counted to ascertain the percent apoptotic cell count for every sample. Experiment was performed three times and the mean and standard deviation of the three experiments were obtained.