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. 2012 Feb 17;7(2):e30604. doi: 10.1371/journal.pone.0030604

Figure 2. DRE induces late stage DNA damage in MV-4-11 cells.

Figure 2

MV-4-11 cells were treated with 1.0 mg/ml DRE and 10 µM VP-16 at the indicated time points to determine if DNA damage was the cause or effect of apoptosis observed in CMML cells. Following treatment, cells were fixed and immunostained with anti-BrdU antibody to observe DNA damage. Increase in TUNEL positive staining corresponds with an increase in levels of DNA damage following treatment. More positively stained cells are observed in the final stages of apoptosis caused by DRE, compared to the DNA targeting drug, VP-16. magnification: 200×.