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. 2012 Feb 17;7(2):e30632. doi: 10.1371/journal.pone.0030632

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Wang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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using examples from murine organotypic brain slice cultures. (A) An example of a focal plane from murine OSC at 40× magnification, showing CIC_α = 0% using our method, (B) side view of the z-stack (view in the x and z plane) for Figure A, (C) view in the y and z plane for Figure A, (D) The same example image as Figure A showing the result using Manders' method, where the white region indicates co-localisation, (E) side view of the z-stack for Figure D, (F) bottom view of the z-stack for Figure D.

Inline graphic — using examples from murine organotypic brain slice cultures. (A) An example of a focal plane from murine OSC at 40× magnification, showing CIC_α = 0% using our method, (B) side view of the z-stack (view in the x and z plane) for Figure A, (C) view in the y and z plane for Figure A, (D) The same example image as Figure A showing the result using Manders' method, where the white region indicates co-localisation, (E) side view of the z-stack for Figure D, (F) bottom view of the z-stack for Figure D.