Skip to main content
. 2012 Feb 17;7(2):e30758. doi: 10.1371/journal.pone.0030758

Figure 2. Melphalan or rTGF-β1 exposure diminished the ability of HOB to support CD34+ bone marrow cells.

Figure 2

A) To evaluate HOB support of CD34+ bone marrow cells, HOB cells were exposed to melphalan [25 µg/ml] or rTGF-β1 [10 ng/ml] for 24 hours. After the 24 hour exposure, the HOB were thoroughly rinsed and CD34+ cells (8.8×105) were added in co-culture. Recombinant IL-3 (100 ng/ml) was added in all groups. CD34+ cells were collected after co-culture for 48 hours and the samples were analyzed for the expression of cell surface markers. B) HOB were exposed to melphalan [50 µg/ml] or rTGF-β1 [10 ng/ml] for 24 hours. The HOB layers were rinsed and CD34+ bone marrow cells (1.75×105) were added in co-culture for 48 hours. The CD34+ cells were collected from the HOB layers, viability determined, and 1×103 viable CD34+ bone marrow cells were added to methocult containing factors for myeloid lineage in triplicate. Colonies were counted and scored after 7 days in culture. *p<0.05 (average number of colonies, treated vs untreated), **p<0.05 (CFU-GM colonies, treated vs untreated) and #p<0.05 (CFU-M colonies, treated vs untreated).