Table 4. Overexpression and suppression of GmBBX52 and GmBBX53 alters yield.
| Construct | Event | Yield (kg/h) | % change vs control | Δ DOF | Δ MAT |
| GmBBX52 overexpression | 1 | 5092 | 3.3 | 1.9** | 4.7** |
| GmBBX52 overexpression | 2 | 5170 | 4.9* | 0.1 | 3.8** |
| GmBBX52 overexpression | 3 | 5304 | 7.7** | −2.7** | 3.5** |
| GmBBX52 overexpression | 4 | 5154 | 4.6* | −0.3 | 3.7** |
| GmBBX52 overexpression | 5 | 5280 | 7.2** | −1.7** | 2.4** |
| GmBBX52 overexpression | 6 | 5307 | 7.7** | 0.4 | 3.1** |
| GmBBX52 overexpression | 7 | 5368 | 9.0** | −1.4** | 3.6** |
| GmBBX52 overexpression | 8 | 5131 | 4.1 | −0.6 | 3.9** |
| GmBBX53 overexpression | 1 | 5023 | 6.7** | −0.3 | 2.4** |
| GmBBX53 overexpression | 2 | 4939 | 4.9** | −0.5 | 1.2* |
| GmBBX53 overexpression | 3 | 4806 | 2.1** | 0.3 | 3.9** |
| GmBBX53 overexpression | 4 | 4844 | 2.9** | 0.02 | 1.3* |
| GmBBX52 miRNA | 1 | 4393 | −11.8** | 0.4 | −1.9** |
| GmBBX52 miRNA | 2 | 4725 | −5.1** | 1.1** | 0.1 |
| GmBBX52 miRNA | 3 | 4757 | −4.5* | 1.1** | 0.4 |
| GmBBX52 miRNA | 4 | 4807 | −3.4 | −0.3 | 0.4 |
| GmBBX52 miRNA | 5 | 4674 | −6.1** | 2.8** | 2.0** |
| GmBBX52 miRNA | 6 | 4910 | −1.4 | −0.8 | 0.1 |
| GmBBX52 miRNA | 7 | 4812 | −3.3 | 0.2 | −0.04 |
| GmBBX52 miRNA | 8 | 4556 | −8.5** | −0.3 | −0.1 |
Analysis of individual lines from one season of field testing of soybean plants overexpressing the soybean homologs of AtBBX32, GmBBX52 and GmBBX53, demonstrate that the expression of the soybean genes leads to significant yield improvements relative to control. miRNA mediated knockdown of GmBBX52 leads to decreased yield relative to control. The difference in the days to flower (DOF) between the transgenic lines and control was calculated to determine delta DOF. The difference in days to final maturity (MAT) was examined in transgenic lines and compared to control to determine delta MAT (units = days). p-values were based on the difference between the transgenic lines and wildtype control.
**p≤0.05,
*p≤0.1.