Figure 7. Differentiation of hematopoietic precursors in direct contact with CFU-Fs from irradiated (black) and non-irradiated (grey) bone marrows.

A: Immunophenotyping shows a myelo-monocytic (CD11b), a granulocytic (Ly-6G) and a B-lymphoid (CD45R/B220) engagement of hematopoietic precursors in lineages. B: The differentiation of mature neutrophils during the co-culture assay occurs earlier when precursors are co-cultured on “day 3” CFU-Fs. C: Inhibition of the contact in transwell conditions induces a significant reduction in the differentiation of the Gr1+ (Ca) and CD11b+ (Cb) lineages. Data are presented as mean values ± SEM of at least three independent experiments. **, p<.01; *, p<.05 as assessed by one way analysis of variance (ANOVA).