Table 1. Binding affinities of single mutants for MD2.
| Mutant | Ka (M−1 S−1) | Kd (S−1) | KD (M) | Fold-increase |
| Wild-type | 2.84±0.02×104 | 2.22±0.05×10−3 | 7.80±0.14×10−8 | 1 |
| M41E | 1.64±0.57×104 | 5.29±0.80×10−5 | 3.21±1.02×10−9 | 24.3 |
| F63L | 2.65±0.02×104 | 1.15±0.01×10−3 | 4.33±0.03×10−8 | 1.8 |
| F63W | 1.39±0.10×104 | 3.97±2.13×10−5 | 2.84±1.66×10−9 | 27.5 |
| V132F | 6.91±3.69×104 | 1.54±0.01×10−3 | 2.22±1.41×10−8 | 3.5 |
| V134L | 2.67±0.28×104 | 1.10±0.01×10−4 | 4.11±0.50×10−9 | 19.0 |
| N156I | 1.97±0.33×104 | 8.05±4.77×10−5 | 4.07±1.71×10−9 | 19.2 |
| H159Q | 2.14±1.21×103 | 1.00±0.44×10−5 | 4.30±3.23×10−9 | 18.1 |
| D181E | 1.91±0.10×104 | 9.24±1.35×10−4 | 4.82±0.44×10−8 | 1.6 |
| S184K | 7.66±0.02×103 | 1.41±0.02×10−3 | 1.83±0.04×10−7 | 0.4 |
Binding affinities (KD) of the single mutants for MD2 were measured from the association rate constants (Ka) and dissociation rate constants (Kd) using surface plasmon resonance. Fold-increase represents the ratio of binding affinities between the mutants and wild-type decoy receptor.