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. 2012 Feb 17;7(2):e31056. doi: 10.1371/journal.pone.0031056

Figure 7. AMPK mediates OA-NO2-induced eNOS phosphorylation and NO production in BAECs.

Figure 7

BAECs were treated with (A) different concentrations of OA-NO2 for 16 h or (B) 2.5 µmol/L OA-NO2 for the indicated times. Lysates were analyzed by western blot for the indicated proteins. The blot is representative of three blots obtained from three separate experiments. C) Western blot of phosphorylated AMPK and eNOS in OA-NO2-stimulated BAECs infected with Ad-DN-AMPK (MOI = 50). Non-infected cells or cells infected with Ad-GFP served as controls. For A–C, the corresponding densitometric analyses are shown. *p<0.05 vs. control; # p<0.05 vs. GFP with OA-NO2-treated group. D) NO release by OA-NO2-stimulated BAECs infected with Ad-DN-AMPK (MOI = 50) or Ad-GFP (control). *p<0.05 vs. non-transfected, no OA-NO2 group; # p<0.05 vs. OA-NO2-treated, Ad-GFP group. E) AMPK activity corresponding to C and D above. *p<0.05 vs. no OA-NO2 treatment, Ad-GFP group; # p<0.05 vs. OA-NO2-treated, Ad-GFP group. F) The proposed signaling pathway involved in AMPK/eNOS activation in response to OA-NO2 treatment in endothelial cells.