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. 2012 Feb 17;7(2):e31285. doi: 10.1371/journal.pone.0031285

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Yoshimura et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, Black rice section stained with toluidine blue O after MALDI-IMS analysis of the ions at m/z 449. B, Localization pattern of the ions at m/z 449. C, Localization pattern of the ions at m/z 822 corresponding to PC (diacyl C36:3), which marks the nucellus epidermis/aleurone layer. D, Localization pattern of the ions at m/z 496 corresponding to LPC (C16:0), which marks the endosperm. E, Merged ion image of m/z 449 (red), m/z 822 (green) and m/z 496 (white). F, Black rice section stained with toluidine blue O after MALDI-IMS analysis of the ions at m/z 463. G, Localization pattern of the ions at m/z 463. H, Localization pattern of the ions at m/z 822 corresponding to PC (diacyl C36:3). I, Localization pattern of the ions at m/z 496 corresponding to LPC (C16:0). J, Merged ion image of m/z 463 (red), m/z 822 (green) and m/z 496 (white). Scale bar: 1.0 mm.