Figure 5. T cell derived IFNγ is necessary for protection from bleomycin induced fibrosis.

A. Survival curves of wild type and Rag null mice following vaccinia vaccination or mock (PBS) vaccination and bleomycin administration. B. Flow cytometric analysis of T cell reconstitution of Rag null mice. C. Survival curves of Rag null mice reconstituted with either wild type of IFNγ null CD4⁺ and CD8⁺ T cells. All mice were vaccinated with vaccinia and bleomycin was administered. D. Flow cytometric analysis of lung CD4⁺ IFNγ⁺ and CD4⁺ IL17⁺ T cells. E. Flow cytometric analysis of lung CD11B⁺ IFNγ⁺ cells. Histograms are gated on CD11b⁺ cells. All experiments were performed at least three times, at least 10 animals per group per experiment.