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. 2012 Feb 17;7(2):e31431. doi: 10.1371/journal.pone.0031431

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Zhu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) mRNA levels of SIRT1 in LV-Vector and LV-ATF4 stably transfected SGC7901 cell lines (left) and LV-SCR and LV-siATF4 stably transfected SGC7901/ADR cells (right) were subjected to qPCR. GAPDH were used as an internal control. Data represent the means ± S.D. of three independent experiments. (B) Cell lysates from cells in section A and their respective nontreated counterparts(NC) were blotted with the indicated antibodies. β-actin was used as an internal control.