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. 2012 Feb 17;7(2):e31431. doi: 10.1371/journal.pone.0031431

Figure 6. Inhibition of SIRT1 activity reintroduce sensitivity in ATF4-overexpressing cell lines.

Figure 6

(A) LV-Vector and LV-ATF4 stably transfected SGC7901 cell lines were incubated with or without the indicated doses of EX-527. Ninety-six hours later, cell viabilities were determined by MTT assay. (B) Stably transfected SGC7901 cell lines in section A were incubated with or without EX-527 (1–10 µM) for 24 h, and total cell lysates were subjected to immunoblotting with the indicated antibodies. β-actin was used as an internal control. (C) SGC7901-ATF4 cells were preincubated with the indicated doses of EX-527 for 24 h. Then SGC7901-Vector and SGC7901-ATF4 cells were exposed to cisplatin (1 µg/ml) or 5-fluorouracil (1.25 µg/ml) for additional 72 h. Cell viabilities were determined by MTT assay. (D) SGC7901-ATF4 cells were preincubated with or without EX-527 (10 µM) for 24 h. Then the cells were exposed to the indicated doses of cisplatin or 5-fluorouracil for additional 72 h. Cell viabilities were determined by MTT assay. All data represent the means ± S.D. of three independent experiments. Graphs provide average quantification as a percentage of the nontreated cells.