Figure 2.

ChIP-Seq Demonstrates RING1B Occupancy at PcG Target Loci in Eed^+/+ and Eed^−/− mESCs

(A) Example screen shots for Eed^+/+ IP (blue) and Eed^−/− IP (red) and input (lilac). Irx2 and Msx1 are PcG targets. Oct3/4 and Gata1 are non-PcG targets.

(B) Venn diagram indicating overlap of RING1B peaks (genes) in Eed^+/+and Eed^−/− cells.

(C) Tag density in relation to TSS of 20 million reads randomly subsampled.

(D) Comparison showing similarity in gene ontology distribution for peaks in Eed^+/+ and Eed^−/− mESCs.

(E) Co-incidence of peaks with CpG islands for Eed^+/+ and Eed^−/− mESCs (above) and the proportion of peaks coinciding with CpG islands in overlapping and nonoverlapping subgroups defined in (B).

See also Figure S2.