Figure 4.

Proteomic Analysis of MEL-18, RYBP, and CBX7 Complexes in mESCs

(A) Silver-stained SDS polyacrylamide gel of control, MEL-18-Flag, RYBP-Flag, and CBX7-Flag purifications. PRC1 subunits identified by mass spectrometry of excised bands are indicated.

(B) Table showing the PRC1 core subunits copurifying with MEL-18-Flag, RYBP-Flag, and CBX7-Flag, as identified by mass spectrometry analysis. ¹Mascot score for specified proteins, ²number of unique peptides identified. ^∗The two peptides matched to RING1A are also present in RING1B.

(C) MEL-18-Flag, RYBP-Flag, and CBX7-Flag purifications analyzed by western blot with the indicated antibodies.

See also Figure S4.