Figure 3.

DFTD Origin

(A) Y chromosome gene SRY is not detectable in DFTD using PCR. Primer sequences are available (Lachish et al., 2011; Murchison et al., 2010).

(B) Number of X chromosome variants in female and male normal devil genomes and 87T and 53T DFTD genomes. Variants from a poorly assembled region at the end of chromosome X were excluded from this analysis.

(C) Phylogenetic tree of devil mitochondrial variation. Each dot on the map indicates an individual devil and the color of the dot represents the mitochondrial haplotype for each devil. Each haplotype is also represented on the phylogenetic tree. DFTD mitochondrial haplotypes are indicated in gray; some DFTD tumors also had the haplotype represented by the red dot.

See also Figure S3 for chromosome X copy number plots for 87T and 53T.