Skip to main content
. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Circ Cardiovasc Genet. 2012 Feb 1;5(1):o8–o18. doi: 10.1161/CIRCGENETICS.111.960187

Figure 2.

Figure 2

Schematic workflow and MRM analysis of one peptide unique to the target protein ST2. The ST2 protein was digested with trypsin and the resulting peptides were analyzed using a triple quadrupole MS instrument. Peptides were targeted within the MS instrument and only those peptides selected based on their mass (parent ion) were analyzed. The specificity was obtained based on the peptides elution times from the online RPLC and the corresponding MS/MS data (transitions). Three transitions are shown for one selective parent peptide (ITDFGEPR) that is unique for the protein ST2 (accession # Q01638; ILRL1_HUMAN). The MS/MS data provides amino acid sequence information and ensures a high level of specificity. Three peptides and two transitions per peptide are commonly used for quantification.