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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Eur J Oral Sci. 2011 Dec;119(Suppl 1):206–216. doi: 10.1111/j.1600-0722.2011.00866.x

Fig. 3.

Fig. 3

Kallikrein-related peptidase 4 (KLK4) in Mmp20 null mice. Kallikrein-related peptidase 4 elutes from a reverse-phase high-performance liquid chromatography (RP-HPLC) column between 50 and 52.5 min. Left: enamel proteins (1.2 mg) from wild-type, Mmp20 null, and Klk4 null mice were fractionated by RP-HPLC and three fractions were collected, from (i) 45–50 min, (ii) 50.0–52.5 min, and (iii) 52.5–55.0 min. Right: zymography and western blot analyses of the three fractions. Active KLK4 was detected in the Mmp20 null mice migrating as a doublet. An unknown proteolytic activity migrating at 40 kDa was observed only in the Mmp20 null mice. Triangles indicate KLK4 bands; the arrowhead marks an unidentified 40-kDa protease.