. Author manuscript; available in PMC: 2013 Feb 14.

Published in final edited form as: Biochemistry. 2012 Jan 30;51(6):1134–1147. doi: 10.1021/bi201206v

Table 11. Nitrate-reducing activity of the CSO variants and wild-type NR proteins.

	K_m^nitrate (μM)	k_cat (s⁻¹)	k_cat/K_m^nitrate (M⁻¹s⁻¹)	Reference
pH 8.5:
CSO^*	>20000	<0.15	ND	This work
CSO-2NR	1134	0.751	6.6×10²	This work
CSO-3NR	151.1	0.377	2.4×10³	This work
pH 7.0:
CSO^*	>20000	<0.10	ND	This work
CSO-2NR	445	0.65	1.4×10³	This work
CSO-3NR	72.7	0.96	1.3×10⁴	This work
S. oleracea	12	180	1.5×10⁷	Pollock et al. 2002(39)
A. thaliana	90	100	1.1×10⁶	Skipper et al. 2001(24)
P. pastoris	30	159	5.3×10⁶	Barbier et al. 2004(38)

ND Not determined