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Stopped flow analysis was carried out anaerobically as described in Experimental Procedures
| kred(s−1) | Kdsulfite(μM) | |
|---|---|---|
| pH 8.5 | ||
| Wild type | 82.8 | 6.72 |
| R472Q | 71.2 | 227 |
| R472M | 38.9 | 73.4 |
| pH 7.0 | ||
| Wild type | 70.2 | 2.05 |
| R472Q | 83.4 | 14.5 |
| R472M | 39.0 | 3.23 |
