Table 4. Steady state activity of the Mo domains of wild-type, R472Q, and R742M SO using Ferricyanide as an artificial electron acceptor.
Vmax (U/mg) | Kmsulfite(μM) | |
---|---|---|
pH 8.5 | ||
Wild type | 99.8 | 14.9 |
R472Q | 99.6 | 223 |
R472M | 46.4 | 94.0 |
pH 7.0 | ||
Wild type | 78.0 | 4.56 |
R472Q | 50.5 | 12.8 |
R472M | 27.9 | 5.00 |