. Author manuscript; available in PMC: 2013 Feb 14.

Published in final edited form as: Biochemistry. 2012 Jan 30;51(6):1134–1147. doi: 10.1021/bi201206v

Table 5. K_m^sulfite values of active site HSO variants.

pH	Wild type K_m (μ)	Y343N K_m (μM)	V474M K_m (μM)	Y343F/R472Q K_m (μM)	Y343N/R472M K_m (μM)	Y343N/R472M/V474M K_m (μM)
6.0	1.29	95.3	0.46	61.4	16800	1100
7.0	2.72	94.7	1.34	87.7	4640	1420
8.0	4.35	297	3.19	282.7	19280	2140
8.5	8.25	850	3.54	712.5	42990	14000
9.0	22.1	2460	14.4	3340	85640	55500
9.5	67.1	9370	31.9	39900	208000	111000
10.0	52.9	12013	40.2	59660	ND^*	418000

Not Determined

Table 5. Kmsulfite values of active site HSO variants.