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| pH | Wild type kcat (s−1) |
Y343N kcat (s−1) |
V474M kcat (s−1) |
Y343F/R472Q kcat (s−1) |
Y343N/R472M kcat (s−1) |
Y343N/R472M/V47M kcat (s−1) |
|---|---|---|---|---|---|---|
| 6.0 | 13.2 | 3.17 | 5.96 | 1.44 | 1.35 | 1.90 |
| 7.0 | 24.2 | 12.77 | 11.4 | 1.73 | 1.13 | 3.37 |
| 8.0 | 25.9 | 13.75 | 17.8 | 2.05 | 1.33 | 3.58 |
| 8.5 | 26.9 | 16.91 | 15.8 | 2.25 | 1.42 | 3.90 |
| 9.0 | 25.7 | 15.54 | 19.6 | 2.31 | 1.13 | 5.60 |
| 9.5 | 26.3 | 8.11 | 17.1 | 4.94 | 0.97 | 5.23 |
| 10.0 | 13.0 | 4.38 | 12.4 | 2.96 | ND* | 2.80 |
Not Determined
