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. Author manuscript; available in PMC: 2012 Jun 8.
Published in final edited form as: Neuron. 2011 Dec 8;72(5):721–733. doi: 10.1016/j.neuron.2011.10.028

Figure 4. Specificity and functionality of Chat::Cre rats.

Figure 4

A. Quantification of YFP expression profile in Chat::Cre sublines in the medial septum (MS) nucleus basalis (NB) and nucleus accumbens (NAc) after injection of a Cre-dependent virus. Errorbars are SEM. B. Colocalization of ChAT staining and YFP expression in the MS (top), NB (middle), and NAc (bottom). C. Membrane potential changes induced by current injection in a ChR2-YFP expressing ChAT neuron. VM = −53 mV. Current steps: −100, +50 pA. D. Voltage clamp recording of a neuron expressing ChR2-YFP in slice showing inward current in response to blue light (1 sec constant illumination, 470 nm). E. Current clamp recording of light-evoked action potentials in a ChAT neuron expressing ChR2-YFP in slice in response to several stimulation frequencies (470 nm, 2 ms pulse duration, 40 pulses). F. In vivo inhibition of multiunit activity in response to optical activation of ChAT cells in the nucleus basalis with ChR2. Top: example voltage trace. Middle: Raster plot from 8 repetitions. Bottom: Time average and SEM (from raster plot). (10 Hz, 10 ms pulse duration, 5 pulses, 473nm)