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. Author manuscript; available in PMC: 2012 Feb 18.
Published in final edited form as: Methods Enzymol. 2001;339:285–313. doi: 10.1016/s0076-6879(01)39319-9

Fig. 11.

Fig. 11

(A) Solution NMR 1H chemical shift/15N chemical shift correlation HMQC spectrum of AchR M2 in DPC micelles. (B) Solid-state NMR 1H–15N dipolar/15N chemical shift correlation PISEMA spectrum of AchR M2 in oriented lipid bilayers. (C) Superposition of the backbone heavy atoms for the 10 lowest energy structures of AchR M2 in DPC micelles. (D) Superposition of the average structure of the AchR M2 calculated from the solution NMR distance constraints (light), and the average structure determined from the solid-state NMR orientational constraints (dark). (E) Side and (F) top views of the AchR M2 pentameric bundle in the funnel-like architecture determined by solid-state NMR spectroscopy. The wide mouth of the funnel is on the N-terminal, intracellular side of the pore. The side view has the C terminus on top. The top view has the C-terminal synaptic side in front. A sodium ion is confined within the pore.