Figure 4. ANXA2 depleted cells show enhanced activation of oxidative stress responsive signaling proteins.

(A, B) MCF7 ANXA2 shRNA1, MCF7 ANXA2 shRNA2 or MCF7 ANXA2 scramble cells were treated with (A) 1 mM H₂O₂ for the times indicated or (B) 1 mM H₂O₂ in the absence or presence of 10 mM NAC for 30 min; (C) A549 ANXA2 shRNA2 or A549 ANXA2 scramble cells were treated with 1 mM H₂O₂ in the absence or presence of 10 mM NAC for the times indicated; (D) LLC ANXA2 shRNA3 or LLC ANXA2 scramble cells were treated with 1 mM H₂O₂ for 30 minutes. (A-D) 25 μg of each cell lysate was subjected to SDS-PAGE followed by western blotting with the antibodies indicated.