Figure 2. TAp73alpha expression inhibits caspase-2 activity.

SCLC NCI-H82 cells (Figure 2) were transfected with TAp73alpha, caspase-2 and/or empty expression vectors, and caspase-2 activity was monitored. The enzyme activity of caspase-2 (VDVADase activity) was evaluated as the release of AMC from the substrate Ac-VDVAD-AMC (Ac-Val-Asp-Val-Ala-Asp-7-amino-4-methylcoumarin). The data represents three independent experiments.