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. 2011 Dec 30;2(12):1254–1264. doi: 10.18632/oncotarget.406

Figure 2. Schematic of the array protocol followed to readout the relative abundance of individual shRNAs following treatment.

Figure 2

The barcoded shRNA inserts from mock or drug treated cells are labeled with Cy3-nucleotides and used as probes in a competitive two-color hybridization with Cy5-labeled probe generated from the barcoded shRNA inserts amplified from the E. coli generated library.