Table 1. Functional classification of the 97 genes associated with response to GSK461364A.
The DAVID Gene Functional Classification Tool was used to analyze the 97 genes targeted by the 201 shRNAs associated with response to GSK461364A. The resultant groupings and 50 genes are listed here, together with the average shRNA relative abundance (drug treatment vs. mock treatment) on a log2 scale.
| Grouped Genes | Description | shRNA effect |
|---|---|---|
| Cell surface receptor linked signal transduction | ||
| CSF1R | colony stimulating factor 1 receptor | −0.30 |
| DDR1 | discoidin domain receptor tyrosine kinase 1 | −0.41 |
| EGFR | epidermal growth factor receptor | −0.50 |
| GUCY2D | guanylate cyclase 2D, membrane | −0.46 |
| IGF1R | insulin-like growth factor 1 receptor | −0.28 |
| KIT | proto-oncogene tyrosine-protein kinase Kit | −0.45 |
| TGFBR1 | transforming growth factor, beta receptor 1 | −0.54 |
| TIE1 | tyrosine kinase with immunoglobulin-and EGF-like domains | −0.52 |
| Protein kinase | ||
| BMX | BMX non-receptor tyrosine kinase | −0.86 |
| CAMKK1 | calmodulin-dependent protein kinase kinase | −0.86 |
| CDC7 | cell division cycle 7 homolog (S. cerevisiae) | −0.39 |
| CDK7 | cyclin-dependent kinase 7 | −0.34 |
| CSF1R | colony stimulating factor 1 receptor | −0.29 |
| CSNK1G1 | casein kinase 1, gamma 1 | −0.55 |
| CSNK2A2 | casein kinase 2, alpha prime polypeptide | −0.44 |
| DDR1 | discoidin domain receptor tyrosine kinase 1 | −0.41 |
| DYRK4 | dual-specificity tyrosine-(Y)-phosphoryl. regulated kinase 4 | −0.47 |
| EIF2AK2 | eukaryotic translation initiation factor 2-alpha kinase 2 | −0.25 |
| ICK | intestinal cell (MAK-like) kinase | −0.42 |
| LATS2 | LATS, large tumor suppressor, homolog 2 (Drosophila) | −0.54 |
| MAP2K6 | mitogen-activated protein kinase kinase 6 | 0.61 |
| MAP2K7 | mitogen-activated protein kinase kinase 7 | −0.23 |
| MAP3K13 | mitogen-activated protein kinase kinase kinase 13 | 1.11 |
| MAPK10 | mitogen-activated protein kinase 10 | −0.87 |
| MAPK14 | mitogen-activated protein kinase 14 | −0.16 |
| MAPK15 | mitogen-activated protein kinase 15 | −0.39 |
| MAST4 | microtubule associated serine/threonine kinase 4 | −0.82 |
| PFKP | phosphofructokinase, platelet | 0.46 |
| SCYL3 | SCY1-like 3 (S. cerevisiae) | −0.86 |
| SGK3 | serum/glucocorticoid regulated kinase 3 | 0.24 |
| SLK | STE20-like kinase (yeast) | −0.36 |
| TAOK2 | TAO kinase 2 | −0.87 |
| TIE1 | tyrosine kinase with immunoglobulin-and EGF-like domains | −0.52 |
| M-phase of mitotic cell cycle | ||
| ANAPC5/APC5 | anaphase promoting complex subunit 5 | −0.36 |
| CDC16 | cell division cycle 16 homolog (S. cerevisiae) | −0.53 |
| CDC20 | cell division cycle 20 homolog (S. cerevisiae) | −0.56 |
| CDC27 | cell division cycle 27 homolog (S. cerevisiae) | −0.44 |
| Nuclear hormone receptor, ligand-binding | ||
| NR1I3 | nuclear receptor subfamily 1, group I, member 3 | −1.28 |
| NR2C1/TR2 | nuclear receptor subfamily 2, group C, member 1 | −0.75 |
| NR2C2/TR4 | nuclear receptor subfamily 2, group C, member 2 | −0.65 |
| NR2E3 | nuclear receptor subfamily 2, group E, member 3 | −0.65 |
| NR3C2 | nuclear receptor subfamily 3, group C, member 2 | −0.47 |
| NR4A2/NURR1 | nuclear receptor subfamily 4, group A, member 2 | −1.39 |
| NR4A3/NOR1 | nuclear receptor subfamily 4, group A, member 3 | 0.71 |
| NR5A1 | nuclear receptor subfamily 5, group A, member 1 | −0.87 |
| RARA | retinoic acid receptor, alpha | 0.62 |
| Protein-tyrosine phosphatase | ||
| DCC | deleted in colorectal carcinoma | 0.79 |
| PTPRD | protein tyrosine phosphatase, receptor type, D | −0.72 |
| PTPRF | protein tyrosine phosphatase, receptor type, F | −0.64 |
| PTPRG | protein tyrosine phosphatase, receptor type, G | −0.47 |