Figure 4. Fzd6 expression is associated to activation of the non-canonical Wnt pathway.

A) Fzd6 positive and negative cells were subjected to immunofluorescence staining with an antibody specific for activated beta-catenin (indicated by ABC). The average of the percentages of cells positive for the antibody in multiple fields are plotted in the y axis. The error bars indicate standard deviations. The difference is not statistically significant (p=0.1). B) western blot analysis showing the expression of total (left panel) and activated/phospho (right panel) JNK in Fzd6 positive (+) and negative (−) neuroblastoma cells.