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. Author manuscript; available in PMC: 2012 Feb 18.
Published in final edited form as: Nat Protoc. 2010 Apr 1;5(4):758–772. doi: 10.1038/nprot.2010.38

Figure 1.

Figure 1

Standard Golgi disassembly and reassembly assay in which purified Golgi membranes were treated with mitotic and interphase HeLa cell cytosol (a,b). (a) Purified rat liver Golgi membranes were (b) first treated with mitotic HeLa cell cytosol at 37 °C for 20 min. (c) Membranes were reisolated by centrifugation and subsequently treated by interphase cytosol at 37 °C for 60 min. Membranes were fixed and processed for EM. Scale bar, 0.5 μm. Note that treatment with mitotic cytosol led to fragmentation of the Golgi membranes, whereas further treatment of the Golgi fragments with interphase cytosol allowed the reassembly of new Golgi stacks. Reprinted and modified from Tang et al.21 with permission from the journal. Our plan for animal use is approved by the University of Michigan Committee on Use and Care of Animals (UCUCA, approval number 09146).