. Author manuscript; available in PMC: 2012 Feb 18.

Published in final edited form as: Nat Protoc. 2010 Apr 1;5(4):758–772. doi: 10.1038/nprot.2010.38

TABLE 1.

Components of sucrose gradient buffers for rat liver Golgi preparation.

Buffer	A	B	C	D	E
Sucrose concentration (M)	0	0.25	0.5	0.86	1.3
phosphate buffer (0.5 M, pH 6.7) (ml)	10	10	20	20	10
Sucrose (2 M, ml)	0	6.25	25	43	32.5
MgCl₂(2 M, ml)	0.125	0.125	0.25	0.25	0.125
Water (ml)	39.9	33.6	54.8	36.8	7.4
Total volume (ml)	50	50	100	100	50
Sucrose % (wt/wt)	0	8.6	16.0	26.4	38.6
Refractive index	1.3330	1.3456	1.3574	1.3747	1.3973