Figure 3. Golgin-84 is localized throughout the Golgi stack using immuno-EM.
A. NRK cells were processed for cryo-EM and triple-labeled for Rab1 (5 nm gold), golgin-84 (10 nm gold) and GM130 (15 nm gold). Note the occasional concentration of golgin-84 in cisternal rims (bottom panel). N = nucleus. B. Quantitation showing that rab1 and GM130 are restricted to the cis-side of the Golgi stack (C, C + 1), whereas golgin-84 is present throughout the stack, with the lowest linear density on the cis-side (C).