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. Author manuscript; available in PMC: 2012 Feb 18.
Published in final edited form as: Traffic. 2003 Mar;4(3):153–161. doi: 10.1034/j.1600-0854.2003.00103.x

Figure 3. Golgin-84 is localized throughout the Golgi stack using immuno-EM.

Figure 3

A. NRK cells were processed for cryo-EM and triple-labeled for Rab1 (5 nm gold), golgin-84 (10 nm gold) and GM130 (15 nm gold). Note the occasional concentration of golgin-84 in cisternal rims (bottom panel). N = nucleus. B. Quantitation showing that rab1 and GM130 are restricted to the cis-side of the Golgi stack (C, C + 1), whereas golgin-84 is present throughout the stack, with the lowest linear density on the cis-side (C).