Figure 4. Transient expression of golgin-84 stabilizes the Golgi against disassembly triggered by treatment with BFA.
A. NRK cells were fixed before or after treatment with BFA (5 μg/ml for 30 min) then permeabilized and triple-labeled for golgin-84, rab1 and GM130. B. NRK cells expressing GFP-golgin-84 were fixed before or after treatment with BFA (5 μg/ml for 30 min), then permeabilized and labeled for mannosidase 11 and GM130. Note that cells expressing GFP-golgin-84 the Golgi were more resistant to treatment with BFA treatment than uninjected cells. Asterisks indicate micro-injected cells.