Table 1.
A shortlist of recent publications in kidney transplantation that used proteomics.
| Study (year) | Organ | Phenotype | Study size | Discovery technology | Validation technology |
Biosample used |
Molecules identified (n) |
Interesting molecules | Ref. |
|---|---|---|---|---|---|---|---|---|---|
| Freue et al. (2010) | Kidney | Acute rejection | AR = 11, nAR = 21 |
iTRAQ-MALDI-TOF/TOF | ELISA | Plasma | n = 18 | TTN, KNG1, LBP, VASN, AFM | [82] |
| Kurian et al. (2009) | Kidney | Chronic allograft nephropathy | noCAN = 18, mild CAN = 15, severe CAN = 9 |
Shotgun LC/MS/MS | No validation | Peripheral blood lymphocytes | Mild CAN = 302, severe CAN = 509 |
No specific candidate proteins reported; however, 50 protein/mRNA matches were used in a discrimination analysis | [83] |
| Narkorchevsky et al. (2010) | Kidney | Chronic allograft nephropathy | noCAN = 8, mild CAN = 9, severe CAN = 15 |
Shotgun LC/MS/MS | SRM | Tissue biopsy | Unique = 492, differential expression = 904 |
No specific core molecules, but lists pathways/biological functions of interest: complement, actin cytoskeleton and lymphocyte/macrophage cell signaling | [34] |
| Sigdel et al. (2010) | Kidney | Acute rejection | 40 (92 samples) | Shotgun LC/MS/MS | ELISA | Urine | 1446 | UMOD, SERPINF1, CD44 | [20] |
| Dai et al. (2008) | Kidney (rat) | Acute rejection | 18 | MALDI-TOF-TOF-MS | No validation | Tissue | 11 | Chain D, rat transthyretin, apolipoprotein A-IV and Rho GDP | [84] |
| Jahnukainen et al. (2006) | Kidney | BK | 78 | SELDI-TOF-MS | No validation | Urine | A number of peptide peaks | [85] | |
| Quintana et al. (2009) | Kidney | CAN | 71 | LC-ESI-MS | MRM | Urine | A number of peptides | UMOD, kininogen | [86] |
| Ling et al. (2010) | Kidney | Acute rejection | 70 | LC-MS-MS | MRM | Urine | 40 peptides for AR | COL1A2, COL3A1, UMOD, MMP7, SERPING1 and TIMP1 | [19] |
| Metzger et al. (2011) | Kidney | Acute rejection | AR = 44 nAR = 59 |
CE-MS | CE-MS on blinded samples | Urine | A panel of peptides | COL α(I), α(III) chain fragments, MMP8 | [87] |
AFM: Afamin precursor; AR: Acute rejection; BK: BK virus nephropathy; CAN: Chronic allograft nephropathy; CE: Capillary electrophoresis; COL: Collagen; iTRAQ: Isobaric tags for relative and absolute quantitation; KNG1: Kininogen-1; LBP: Lipopolysaccharide-binding protein precursor; LC: Liquid chromatography; MMP: Matrix metalloproteinase; MRM: Multi-reaction monitoring; MS: Mass spectrometry; nAR: Non-acute rejection; noCAN: No chronic allograft nephropathy; SELDI: Surface-enhanced laser desorption ionization; SERPIN: Serine protease inhibitor; SRM: Selected reaction monitoring; TIMP1: Tissue inhibitor of metalloproteinase 1; TTN: Titin; UMOD: Uromodulin; VASN: Vasorin precursor.