Table 2. Sex-specific liver gene expression in global STAT5a-deficient male and female mice.
qPCR analysis was carried out using cDNA prepared from total liver RNA isolated from STAT5a wild-type male (n=6), STAT5a-deficient male (n=8), STAT5a-wild type female (n=4) and STAT5a-deficient female mice (n = 4). Data shown represent relative RNA levels, mean ± S.E. normalized to the 18S rRNA content of each sample and set to 1.0 for wild type male or female liver, as indicated. Statistical differences, determined by one-way ANOVA with Bonferroni post hoc test, represent the following comparisons: STAT5a male vs. STAT5a female (+ = p<0.05 and ++ = p<0.01). None of the genes displayed a significant difference in expression at p<0.05 between STAT5a-wild type and STAT5a-deficient male or female liver. Sex-specificity and class of each gene is represented as male (M) or female (F) and I or II.
| Gene | Sex specificity and Class | STAT5a +/+ Male | STAT5a -/- Male | STAT5a +/+ Female | STAT5a -/- Female |
|---|---|---|---|---|---|
| 18S rRNA | 1.00 ± 0.04 | 0.94 ± 0.02 | 0.95_± 0.02 | 1.00_± 0.06 | |
| Cyp4a12 | MI | 1.00 ± 0.27 | 1.20 ± 0.34 | ≤0.01 + | ≤0.01 + |
| Gst π | MI | 1.00 ± 0.41 | 1.10 ± 0.43 | 0.09 ± 0.06 | 0.20 ± 0.08 |
| Slp | MI | 1.00 ± 0.21 | 0.55 ± 0.11 | 0.02_± 0.01 | 0.01 ± 0.00 |
| Elovl3 | MI | 1.00 ± 0.34 | 0.56 ± 0.17 | ≤0.01 + | 0.01 ± 0.00 |
| Moxd1 | MI | 1.00 ± 0.66 | 0.85 ±0.57 | 0.01_± 0.00 | 0.01 ± 0.00 |
| Mup3 | MII | 1.00 ± 0.20 | 0.83 ± 0.17 | 0.08 ± 0.03 ++ | 0.24 ± 0.04 + |
| Cyp7b1 | MII | 1.00 ± 0.23 | 0.53 ± 014 | 0.07 ± 0.02 + | 0.08 ± 0.01 + |
| Hsd3b5 | MII | 1.00 ± 0.15 | 1.38 ± 0.37 | ≤0.01 ++ | ≤0.01 + |
| Slco1a1 | MII | 1.00 ± 0.25 | 0.57 ± 0.11 | 0.10 ± 0.01 + | 0.16 ± 0.07 + |
| Cyp2d9 | MII | 1.00 ± 0.41 | 1.50 ± 0.37 | 0.05 ± 0.03 ++ | 0.02 ± 0.00 + |
| Mup1/2/6/8 | MII | 1.00 ± 0.17 | 0.97 ± 0.20 | 0.15 ± 0.09 ++ | 0.45 ± 0.38 |
| Cyp2b9 | FI | ≤0.01 | ≤0.01 | 1.00 ± 0.29 ++ | 0.37± 0.11 ++ |
| Cyp2b13 | FI | ≤0.01 | ≤0.01 | 1.00 ± 0.54 + | 0.41 ± 0.25 + |
| Cyp2a4 | FI | 0.21 ± 0.07 | 0.18 ± 0.04 | 1.00 ± 0.20 ++ | 0.87 ± 0.23 ++ |
| Cyp39a1 | FI | 0.31 ± 0.09 | 0.55 ± 0.27 | 1.00 ± 0.24 + | 2.13 ± 0.36 |
| Nnmt | FI | 0.37 ± 0.16 | 0.38 ± 0.10 | 1.00 ± 0.42 | 1.85 ± 0.41 ++ |
| Sult1e1 | FI | 0.02 ± 0.01 | 0.04 ± 0.02 | 1.00 ± 0.18 ++ | 1.92 ± 1.42 |
| Cyp3a16 | FII | ≤0.01 | ≤0.01 | 1.00 ± 0.19 ++ | 0.54 ± 0.13 ++ |
| Cyp3a41 | FII | ≤0.01 | ≤0.01 | 1.00 ± 0.17 ++ | 0.78 ± 0.23 ++ |
| Cyp3a44 | FII | ≤0.01 | ≤0.01 | 1.00 ± 0.34 ++ | 0.78 ± 0.14 ++ |