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. Author manuscript; available in PMC: 2012 Feb 19.
Published in final edited form as: Cell Signal. 2010 Jan 13;22(5):770–782. doi: 10.1016/j.cellsig.2010.01.001

Fig. 10.

Fig. 10

Effects of Akt overexpression on Rac1 activation. COSphox cells were transfected similarly as in Figure 9 to express FPR, and P-Rex1 together with Akt WT or Akt KD (A), or with Akt myr alone or in combination with P-Rex1 (B). After fMLF (1 μM) stimulation for the indicated time, Rac1 activation was determined in PBD-GST pull-down assay. Densitometric analysis was performed and relative level of activated Rac1 was determined after normalization against total Rac1 in the same sample. Data were represented as mean±SEM of three independent experiments. *P < 0.05.