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. Author manuscript; available in PMC: 2012 Feb 19.
Published in final edited form as: Cell Signal. 2010 Jan 13;22(5):770–782. doi: 10.1016/j.cellsig.2010.01.001

Fig. 5.

Fig. 5

Comparison of the isolated DH-PH domains to the full-length P-Rex1 in superoxide reconstitution assay. (A) Superoxide production in COSphox cells expressing the FPR and either the full-length P-Rex1 or its isolated DH-PH domains (iDHPH) or the double mutant at E56 and N238. Notice elevated baseline in cells expressing iDHPH at a DNA input of 1 μg (same amount used for full-length P-Rex1). With reduced DNA input (0.4 μg), superoxide generation is barely inducible by fMLF (dotted line in gray). (B) Quantification of the data in (A), without taking protein expression levels into consideration. (C) The level of expression for the various P-Rex1 constructs showing that iDHPH was expressed at much higher level than the full-length P-Rex1. The amount of DNA used in each experiment was shown below (in μg). All experiments were performed for at least 3 times, and representative data are shown. A colored version of panel (A) is available online.