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. Author manuscript; available in PMC: 2012 Feb 19.
Published in final edited form as: Cell Signal. 2010 Jan 13;22(5):770–782. doi: 10.1016/j.cellsig.2010.01.001

Fig. 9.

Fig. 9

Effects of Akt overexpression on P-Rex1-dependent superoxide generation. COSphox cells were transfected to express FPR, together with P-Rex1, Akt1 (Akt WT), a kinase-dead mutant Akt1 (Akt KD), or a myristoylated Akt1 (Aky myr). (A) Comparison of Akt WT and Akt KD for the effect on P-Rex1-dependent superoxide generation. (B) Effect of Akt myr alone and the combined effect of Akt myr and P-Rex1 in superoxide generation. Note a different Y-axis scale than in (A). (C) Quantification of superoxide generation in (A) and (B), based on integrated chemiluminescence produced in the first 20 min after fMLF stimulation. Data are mean ± SEM based on three independent experiments. ** P < 0.01. (D) Expression of P-Rex1 and the WT and mutant Akt in transfected COSphox cells, as determined with Western blotting using an anti-AU5 antibody for the AU5-tagged P-Rex1 and anti-HA antibody for the HA-tagged Akt. Representative data from one of the three experiments are shown. A part of this figure is shown in color in the online version.