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Fraction of N, I and U as a function of changes in ΔG0N→I for (A) ΔG0I→U = 10 kcal/mol and (B) ΔG0I→U= −2 kcal/mol. For (B) shifts in the ΔG0N→I lead to a large increase in the fraction of U. These simulations apply to three-state unfolding equilibria.
