Skip to main content
. Author manuscript; available in PMC: 2013 Feb 17.
Published in final edited form as: FEBS Lett. 2012 Jan 28;586(4):330–336. doi: 10.1016/j.febslet.2012.01.019

Table 1.

Equilibrium unfolding/refolding parameters for γD-crystallin wild-type and mutants at pH 7.2

γD-crystallin Temperature N ⇌ I I ⇌ U
ΔG0N→I m C½ ΔG0I→U m C½
°C kcal/mol kcal/mol/M M kcal/mol kcal/mol/M M
WT 25
37		 6.23 ± 17.9
10.7 ± 18.7		 2.08 ± 3.90
5.46 ± 9.46		 2.74
2.03		 10.4 ± 24.1
10.3 ± 3.88		 3.12 ± 6.77
4.08 ± 1.60		 3.24
2.51
I4F 25
37		 8.50 ± 2.18
5.99 ± 0.78		 5.26 ± 1.39
4.52 ± 0.62		 1.58
1.35		 7.67 ± 1.30
7.56 ± 0.62		 2.48 ± 0.40
2.83 ± 0.23		 3.00
2.65
V76D 25
37		 5.48 ± 0.66
4.36 ± 0.92		 4.71 ± 0.58
5.38 ± 1.08		 1.18
0.79		 7.85 ± 0.77
8.14 ± 0.84		 2.59 ± 0.25
3.07 ± 0.31		 2.96
2.55
I4F/V76D 25
37		 3.96 ± 0.62
2.67 ± 0.54		 5.99 ± 0.83
5.07 ± 0.74		 0.62
0.50		 9.68 ± 1.04
7.37 ± 0.36		 3.24 ± 0.35
2.81 ± 0.13		 2.79
2.59

ΔG0, m, and C½ are the free energy, the denaturant dependence of ΔG0, and the concentration of urea at the transition midpoint, respectively, for the two transitions of γD-crystallin unfolding. ΔG0, m are reported with the associated fit error.