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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Exp Cell Res. 2011 Jul 7;317(16):2384–2389. doi: 10.1016/j.yexcr.2011.06.019

Figure 3. Effects of overexpression of constitutively active Cdc42 on equatorial actin assembly.

Figure 3

(A) Cells were transfected with GFP-actin (control) or both GFP-actin and mRFP-CACdc42 (CACdc42) and were monitored by time-lapse fluorescence optics. Thick actin bundles are observed outside the equator in cells transfected with mRFP-CACdc42 (arrow). Time elapsed in minutes since anaphase onset is shown at the top-right corner of each image. Scale bar represents 10 μm. (B) Cells transiently transfected with GFP-CACdc42 (green; GFP) or control non-transfected cells were stained with rhodamine-phalloidin (red; F-actin). Arrows indicate the position of the cleavage furrow. Three-dimensional reconstructed images are presented. The scale bar represents 10 μm.