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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Nat Neurosci. 2011 Dec 11;15(1):70–80. doi: 10.1038/nn.3000

Figure 7. Astrocyte dialysis with BAPTA regulates GAT-3 in astrocytes.

Figure 7

a. The upper panels show images of an astrocyte in the stratum radiatum. The astrocyte had been dialyzed with Alexa-488 and then processed for GAT-3 staining. The middle panels show images for similar experiments when the astrocyte had been dialyzed with Alexa-488 and BAPTA (13 mM). In this case the colocalisation between Alexa-488 and GAT-3 was reduced because there was less GAT-3 immunostaining in the patched astrocyte. The lower panels show images for slices pretreated with dynasore. b. Summarizes data from experiments such as those shown in panel a. c. Representative traces and cumulative probability plots show that dynasore (100 μM) did not affect mIPSC amplitude or inter-event interval distributions during recordings from interneurons. d. Dynasore pretreatment abolished the ability of astrocyte BAPTA dialysis to reduce mIPSC amplitudes onto interneurons. e. Dynasore pretreatment also abolished the ability of HC 030031 to reduce the mIPSC amplitudes. f. Dynasore pretreatment did not affect the ability of β-alanine (GAT-3 blocker) to reduce the amplitude of mIPSCs onto interneurons. For panels d–f, the mIPSC frequencies are presented in the text (they were not altered). The scale bar for the images is 20 μm (in panel a). Vertical lines are s.e.m.