Table 1.

Properties of P2X2^FLAG–YFP receptors with and without Qd labeling

	P2X2FLAG–YFP	P2X2FLAG–YFP + antibodies	P2X2FLAG–YFP + antibodies + Qds	Tukey's ANOVA
Electrophysiology
EC50 (μm)	24 ± 3	52 ± 9	70 ± 22	p > 0.05
nH	1.3 ± 0.1	1.2 ± 0.1	1.0 ± 0.2	p > 0.05
IATP peak current (−pA/pF)	426 ± 52	322 ± 36	285 ± 46	p > 0.05
n	10	5	7
FRAP
Time constant (s)	14 ± 1	13 ± 1	16 ± 2	p > 0.05
MF (%)	19 ± 1	19 ± 1	19 ± 1	p > 0.05
IMF (%)	42 ± 1	39 ± 1	45 ± 2	p > 0.05
n	21	20	19

There were no differences between the experimental groups for each of the categories as judged by Tukey's ANOVA (see also Fig 3). Comparative electrophysiology data for WT P2X2 is presented in Results and Figure 2.