Fig. 1.

Measurement of Ca²⁺ mobilization in wild-type and RGS4^-/- pancreatic acinar cells. Briefly, pancreatic acinar cells were isolated from WT and RGS4^-/- mice and treated with various concentrations of carbachol (i.e., 0.05 to 100µM). The treatment concentration was then immediately changed to the maximum concentration (i.e., 1 mM). Ca²⁺ mobilization was calculated using the ratio of the calculated area below the graph of the initial carbachol concentration and that of the maximum carbachol concentration (i.e., 1 mM). Intracellular Ca²⁺ concentrations were measured by fluorescence microscopy using fura2-AM. In RGS4^-/- cells (B), a more sensitive and enhanced response was observed than in WT cells (A). Graphical representation of the data is presented in (C). Car, carbachol.