FIG. 7.

Effect of CRBFE on pancreatic duodenal homeobox 1 (pdx-1) and insulin-like growth factor II (Ins2) gene expressions and insulin protein in INS-1 cells. (A) pdx-1 gene expression assessed by quantitative polymerase chain reaction in INS-1 cells treated with different concentrations of CRBFE. (B) Ins2 gene expression in INS-1 cells treated with or without CRBFE. (C) Western blot analysis of insulin protein in INS-1 cells treated with or without CRBFE. Protein density was quantified using Bandscan version 5.0 software, and the relative ratios of insulin protein to actin were calculated. In all cases, the control was set at 1.