FIG. 5.

Quantitative real-time polymerase chain reaction analysis of MDR1, MRP2, and BCRP mRNA in AL-, AR-, or curcumin-treated Caco-2 cells. Caco-2 cells were treated with AL or AR turmerones (50 or 100 μg/mL) or curcumin (14.7 μg/mL) for 24 hours, and RNA was extracted for quantitative real-time polymerase chain reaciotn. Data (cycle threshold, Ct values) were normalized with that of GAPDH and control samples. Data are mean+SD values of three independent experiments. *P<.05, **P<.005, compared with the vehicle control group.