A, Algorithm for classification of hepatitis C virus (HCV)–positive donations as incident or prevalent infections for selection of cases for sequencing. Boxes in gray indicate the donations from which samples were selected for sequencing for this study. B, Histograms showing success of polymerase chain reaction (PCR) relative to viral loads; frequencies of donations from incident and prevalent cases are shown separately. C, HCV subtypes of sequenced samples by incident and prevalent case status. Abbreviations: NAT, nucleic acid testing; RIBA, recombinant immunoblot assay.