Figure 2.

Tumor necrosis factor α (TNF-α) antagonism increases the number of regulatory T cells (Tregs) in the lungs. Wild-type (WT) and CCR5^–/– mice were given a monoclonal antibody to TNF-α or rat immunoglobulin G (IgG) and intranasally infected with 2 × 10⁶ yeasts. At day 7 postinfection, the proportion (A) and absolute number (B) of Tregs were characterized in the lungs by flow cytometry. Tregs were defined as CD4⁺ Foxp3⁺ T cells. Data represent the mean ± SEM (n = 8–12) from 2–3 experiments. **P < .01; ***P < .001.