Table 1.

Inflammatory Cell Populations in Histoplasma capsulatum–Infected Lungs

	Mean No. of Leukocytes (±SEM) × 105
Phenotype	WT + Rat IgG	WT + anti–TNF-α	CCR5–/– + Rat IgG	CCR5–/– + anti–TNF-α
CD4+ T cell	4.8 ± 1.1	14.0 ± 1.8a	4.9 ± 1.5	4.9 ± 1.2
CD8+ T cell	3.9 ± 0.8	14.0 ± 1.7a	3.2 ± 1.0	3.5 ± 0.9
Neutrophil	2.1 ± 0.5	44.1 ± 5.5a	0.8 ± 0.3	13.0 ± 3.2a
Monocyte	3.5 ± 0.7	58.0 ± 7.1a	2.6 ± 0.9	16.2 ± 4.0a
Macrophage	3.6 ± 0.7	60.3 ± 7.5a	2.3 ± 0.7	15.3 ± 3.9a
Dendritic cell	5.6 ± 1.1	6.0 ± 0.7	2.3 ± 0.7	1.6 ± 0.4

WT and CCR5^–/– mice given rat IgG or anti–TNF-α were infected with 2 × 10⁶ yeasts intranasally and lung leukocytes were stained and characterized by flow cytometry at day 7 postinfection. Cell populations were phenotypically characterized by the following surface markers: CD4⁺ T cells were CD3⁺ CD4⁺; CD8⁺ T cells were CD3⁺ CD8⁺; neutrophils were Ly6G^hi CD11b⁺ CD11c^–; monocytes were Ly6C^hi CD11b⁺ CD11c^–; macrophages were Mac3⁺ CD11b⁺; and DCs were I-Ab^hi CD11c⁺. Data represent the mean ± SEM (n = 6–12) from 2–3 experiments.

Abbreviations: IgG, immunoglobulin G; SEM, standard error of the mean; TNF-α, tumor necrosis factor α; WT, wild type.

^aP < .001 vs WT or CCR5^–/– + rat IgG control.