Table 2.
Cytokine Expression in Histoplasma capsulatum–Infected Lungs
| Log10 RQ |
||||
| Cytokine | WT + rat IgG | WT + anti–TNF-α | CCR5–/– + rat IgG | CCR5–/– + anti–TNF-α |
| IL-12 | 1.80 ± 0.08 | 1.63 ± 0.20 | 1.68 ± 0.10 | 1.83 ± 0.10 |
| IFN-γ | 2.78 ± 0.08 | 3.51 ± 0.04c | 2.96 ± 0.06 | 3.37 ± 0.10a |
| GM-CSF | 0.28 ± 0.05 | 0.64 ± 0.05c | 0.26 ± 0.06 | 0.57 ± 0.07b |
| IL-17A | 3.50 ± 0.08 | 4.13 ± 0.06c | 3.89 ± 0.11 | 4.03 ± 0.13 |
| IL-17F | 1.68 ± 0.21 | 2.67 ± 0.14b | 1.86 ± 0.25 | 2.72 ± 0.15b |
| IL-4 | 1.02 ± 0.12 | 1.88 ± 0.16c | 0.79 ± 0.29 | 1.48 ± 0.15a |
| IL-10 | 1.16 ± 0.09 | 1.45 ± 0.07a | 1.21 ± 0.10 | 1.58 ± 0.08b |
RNA was extracted from lung leukocytes from WT and CCR5–/– mice given rat IgG or anti–TNF-α at day 7 postinfection, and cytokine expression was measured by quantitative real-time polymerase chain reaction. Hypoxanthine-guanine phosphoribosyltransferase was used as an endogenous control, and values represent log increase compared with uninfected lungs. Data represent the mean ± SEM (n = 6–12) from 2–3 experiments. aP < .05; bP < .01; cP < .001 vs WT or CCR5–/– plus rat IgG control.
Abbreviations: GM-CSF, granulocyte macrophage colony-stimulating factor; IFN, interferon; IgG, immunoglobulin G; IL, interleukin; RQ, relative quantification; TNF-α, tumor necrosis factor α; WT, wild type.